In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex.

@article{Smith1991InVA,
  title={In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex.},
  author={Harold C. Smith and Shyh Rong Kuo and John W. Backus and Sarah G. Harris and Charles E. Sparks and Janet D. Sparks},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 4},
  pages={1489-93}
}
Specific apolipoprotein B (apoB) mRNA editing can be performed in vitro on apoB RNA substrates. Native gels and glycerol gradient sedimentation have been used to determine the physical properties of the in vitro editing activity in rat liver cytosolic S100 extracts. ApoB RNA substrates were progressively assembled as 27S complexes for 3 hr with similar kinetics as seen for the accumulation of edited RNA. Assembly was not observed on RNAs from apoB deletion constructs that did not support… CONTINUE READING

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