In vitro antifungal activity of miltefosine and levamisole: their impact on ergosterol biosynthesis and cell permeability of dimorphic fungi

  title={In vitro antifungal activity of miltefosine and levamisole: their impact on ergosterol biosynthesis and cell permeability of dimorphic fungi},
  author={Raimunda S{\^a}mia Nogueira Brilhante and {\'E}rica Pacheco Caetano and Rita Amanda Chaves Lima and D{\'e}bora de Souza Collares Maia Castelo Branco and Rosana Serpa and J. S. de Oliveira and Andr{\'e} Jalles Monteiro and Marcos F{\'a}bio Gadelha Rocha and Rossana de Aguiar Cordeiro and Jos{\'e} J{\'u}lio Costa Sidrim},
  journal={Journal of Applied Microbiology},
This study aimed to evaluate the in vitro activity of miltefosine and levamisole against strains of Coccidioides posadasii in the filamentous phase and strains of Histoplasma capsulatum in filamentous and yeast phases. 

Antifungal activity and mechanism of action of dichloromethane extract fraction A from Streptomyces libani against Aspergillus fumigatus

DCEFA may inhibit A. fumigatus growth by targeting fungal cell membrane which results in the leakage of potassium ions and other cellular components, TOS production and final cell death.

In vitro assessment of antifungal, antibacterial, and antiprotozoal drugs against clinical isolates of Conidiobolus lamprauges.

The in vitro activities suggest that the combination of MTF and AZI or DAP are promising candidate therapies for conidiobolomycosis.

Antifungal activity of deferiprone and EDTA against Sporothrix spp.: Effect on planktonic growth and biofilm formation.

It is revealed that iron chelators deferiprone and ethylenediaminetetraacetic acid reduced the growth of planktonic cells of Sporothrix spp.

Future Research Priorities in Fungal Resistance

Important diagnostic and therapeutic advances that address the rise of resistant fungi as well as new public health initiatives that warrant further investigation are highlighted to help curb the spread of these potentially lethal organisms.

Miltefosine Has a Postantifungal Effect and Induces Apoptosis in Cryptococcus Yeasts

The results suggest that MFS increases the plasma membrane permeability in Cryptococcus via an interaction with ergosterol and also affects the mitochondrial membrane, eventually leading to apoptosis, in line with its fungicidal activity.

Potassium iodide and miltefosine inhibit biofilms of Sporothrix schenckii species complex in yeast and filamentous forms.

It is demonstrated that S. schenckii complex forms bioFilms in vitro, and potassium iodide and miltefosine inhibit Sporothrix spp.

Pathogen–Host Interaction of Histoplasma capsulatum: an Update

The latest developments in understanding of H. capsulatum infection lay the foundation for future clinical breakthroughs in prevention, diagnosis, and treatment of this intracellular fungal pathogen.

Miltefosine Against Scedosporium and Lomentospora Species: Antifungal Activity and Its Effects on Fungal Cells

The data obtained in the present study contribute to clarify the dynamics of the interaction between miltefosine and Scedosporium and Lomentospora cells, highlighting its potential use as new antifungal drug in the future.



In Vitro Activities of Miltefosine and Two Novel Antifungal Biscationic Salts against a Panel of 77 Dermatophytes

The susceptibilities of 77 dermatophytes to miltefosine, 1,12-bis(4-pentylpyridinium)dodecane, PYR, itraconazole, terbinafine (TRB), and butenafine (BTF) were compared and MI was more active than ITC.

In vitro activities of isavuconazole against opportunistic filamentous and dimorphic fungi.

The results suggest that isavuconazole is a broad-spectrum antifungal agent, effective against a wide range of moulds in vitro, and relatively uniform in that they showed strong in vitro inhibitory activity against most of the tested fungi.

Synthesis and Antifungal Activity In Vitro of Isoniazid Derivatives against Histoplasma capsulatum var. capsulatum

The study showed that the compound alone is able to reduce the ergosterol content of planktonic cells and can alter the membrane permeability of the fungus, and N′-(1-phenylethylidene)isonicotinohydrazide alone or combined with AMB might be of interest in the management of histoplasmosis.

In vitro inhibitory effect of miltefosine against strains of Histoplasma capsulatum var. capsulatum and Sporothrix spp.

It is demonstrated that MIL has an antifungal effect in vitro against two potentially pathogenic fungi and that more studies should be performed in order to evaluate its applicability in vivo.

In-vitro antifungal activities of sulfa drugs against clinical isolates of Aspergillus and Cryptococcus species.

In vitro susceptibilities of ten clinical isolates, including five strains of Cryptococcus neoformans var. grubii and five strains of Aspergillus fumigatus, were determined against nine sulfa drugs

Ciprofloxacin shows synergism with classical antifungals against Histoplasma capsulatum var. capsulatum and Coccidioides posadasii

Potential alternatives for the treatment of histoplasmosis and coccidioidomycosis are brought, raising the possibility of using CIP as an adjuvant antifungal therapy, providing perspectives to delineate in vivo studies.

Farnesol against Coccidioides posadasii : its effect on growth, ergosterol

The results showed that farnesol exhibited low MICs against all tested strains, indicating the action of this compound on the fungal membrane, and perspectives for the performance of in vivo studies are brought to further elucidate the effects offarnesol on the host cells.

In vitro activity of miltefosine as a single agent and in combination with voriconazole or posaconazole against uncommon filamentous fungal pathogens.

The in vitro susceptibility of miltefosine, as a single agent and in combination with posaconazole or voriconazole, against these pathogens was determined and synergy was most often observed against F. oxysporum and the mucormycetes.

Hexadecylphosphocholine (Miltefosine) Has Broad-Spectrum Fungicidal Activity and Is Efficacious in a Mouse Model of Cryptococcosis

It is concluded that miltefosine has broad-spectrum antifungal activity and is active in vivo in a mouse model of disseminated cryptococcosis and the relatively small inhibitory effect on PLB1 enzyme activities at concentrations exceeding the MIC by 2 to 20 times suggests that PLB 1 inhibition is not the only mechanism of the antif fungus effect.

Effect of alkylphospholipids on Candida albicans biofilm formation and maturation.

Promising antibiofilm activity was displayed by miltefosine and other alkylphosphocholine compounds, which could be considered a putative option for future treatment of candidaemia associated with biofilm formation, although further evaluation in in vivo systems is required.