In vitro angiogenesis on the human amniotic membrane: requirement for basic fibroblast growth factor-induced proteinases

@article{Mignatti1989InVA,
  title={In vitro angiogenesis on the human amniotic membrane: requirement for basic fibroblast growth factor-induced proteinases},
  author={Paolo Mignatti and Ryoji Tsuboi and E L Robbins and Daniel B. Rifkin},
  journal={The Journal of Cell Biology},
  year={1989},
  volume={108},
  pages={671 - 682}
}
The role of basic fibroblast growth factor-(bFGF) induced proteinases in basement membrane (BM) invasion by bovine capillary endothelial (BCE) cells was studied using a quantitative in vitro assay previously described (Mignatti et al., 1986). 125I-iododeoxyuridine-labeled BCE cells were grown for 72 h on the human amnion BM, and cell invasion was determined by measuring the radioactivity associated with the tissue after removal of the noninvasive cell layer. BCE cells were noninvasive under… CONTINUE READING

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