In vitro and ribosome-bound folding intermediates of P22 tailspike protein detected with monoclonal antibodies.


It remains unclear whether polypeptide chains renaturing in vitro from strong denaturants proceed through the same folding pathway as chains released from ribosome within cells. Folding intermediates formed both in vivo and in vitro have been examined using three monoclonal antibodies shown previously to recognize different epitopes of the native P22… (More)


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