In vitro and in vivo pharmacology and pharmacokinetics of a human engineered monoclonal antibody to epithelial cell adhesion molecule.

@article{Ammons2003InVA,
  title={In vitro and in vivo pharmacology and pharmacokinetics of a human engineered monoclonal antibody to epithelial cell adhesion molecule.},
  author={W. Steve Ammons and Robert J. Bauer and Arnold H. Horwitz and Zhi Jian Chen and Eddie Bautista and Harry H Ruan and Marina Abramova and Kristen R Scott and Russell L. Dedrick},
  journal={Neoplasia},
  year={2003},
  volume={5 2},
  pages={146-54}
}
ING-1(heMAb), a Human Engineered monoclonal antibody to epithelial cell adhesion molecule (Ep-CAM), was evaluated for its in vitro and in vivo activity. The dissociation constant of ING-1(heMAb) for binding to Ep-CAM on HT-29 human colon tumor cells was 2 to 5 nM, similar to chimeric ING-1. In antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity assays, ING-1(heMAb) caused a concentration-dependent lysis of BT-20 breast, MCF-7 breast, HT-29 colon, and CACO-2 colon… CONTINUE READING