In vitro and in vivo mutational analysis of the 3'-terminal regions of hepatitis e virus genomes and replicons.

@article{Graff2005InVA,
  title={In vitro and in vivo mutational analysis of the 3'-terminal regions of hepatitis e virus genomes and replicons.},
  author={Judith Graff and Hanh Nguyen and Chaiyan Kasorndorkbua and Patrick G. Halbur and Marisa E St Claire and Robert H. Purcell and Suzanne U. Emerson},
  journal={Journal of virology},
  year={2005},
  volume={79 2},
  pages={1017-26}
}
Hepatitis E virus (HEV) replication is not well understood, mainly because the virus does not infect cultured cells efficiently. However, Huh-7 cells transfected with full-length genomes produce open reading frame 2 protein, indicative of genome replication (6). To investigate the role of 3'-terminal sequences in RNA replication, we constructed chimeric full-length genomes with divergent 3'-terminal sequences of genotypes 2 and 3 replacing that of genotype 1 and transfected them into Huh-7… CONTINUE READING