In vitro and in vivo expression of virulence genes in Vibrio isolates belonging to the Harveyi clade in relation to their virulence towards gnotobiotic brine shrimp (Artemia franciscana).

@article{Ruwandeepika2011InVA,
  title={In vitro and in vivo expression of virulence genes in Vibrio isolates belonging to the Harveyi clade in relation to their virulence towards gnotobiotic brine shrimp (Artemia franciscana).},
  author={Hettipala Arachchige Darshanee Ruwandeepika and Tom Defoirdt and Patit Paban Bhowmick and I Karunasagar and Iddya Karunasagar and Peter Bossier},
  journal={Environmental microbiology},
  year={2011},
  volume={13 2},
  pages={
          506-17
        }
}
Vibrios belonging to the Harveyi clade are pathogenic marine bacteria affecting both vertebrates and invertebrates, thereby causing a severe threat to the aquaculture industry. In this study, the expression of haemolysin, metalloprotease, serine protease, the quorum sensing master regulator LuxR and the virulence regulator ToxR in different Harveyi clade isolates was measured with reverse transcriptase real-time PCR with specific primers. There was relatively low variation in the in vitro… CONTINUE READING
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