In vitro and in vivo characterization of novel 8-methoxy derivatives of chlortetracycline.

@article{Cacciapuoti1987InVA,
  title={In vitro and in vivo characterization of novel 8-methoxy derivatives of chlortetracycline.},
  author={Anthony Cacciapuoti and Eugene L. Moss and Frederick Menzel and Christina Cramer and W. Weiss and David Loebenberg and Roberta S. Hare and George H. Miller},
  journal={The Journal of antibiotics},
  year={1987},
  volume={40 10},
  pages={
          1426-30
        }
}
The in vitro activities of three new 8-methoxychlortetracyclines, Sch 36969, 33256 and 34164 were compared to tetracycline, minocycline and doxycycline. Against aerobic Gram-negative rods Sch 36969 had a geometric mean MIC (GMM) of 4.2 micrograms/ml, about 8-fold more potent than Sch 33256, and similar to all the other compounds. Sch 36969 also had good activity against methicillin-resistant (GMM, 0.21 micrograms/ml) and -susceptible Staphylococci (GMM, 0.14 micrograms/ml), Streptococci (GMM, 0… 
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A new tetracycline antibiotic from a Dactylosporangium species. Fermentation, isolation and structure elucidation.
An actinomycete identified as a Dactylosporangium sp. produces a new tetracycline, 4a-hydroxy-8-methoxychlortetracycline (Sch 34164). The addition of magnesium ions to complex fermentation media
Direct selection of a specifically blocked mutant of Actinomadura brunnea. Isolation of a third 8-methoxy substituted chlortetracycline.
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The antibiotic produced by the blocked mutant has been identified as 8-methoxychlortetracycline (Sch 36969) based upon its biological activity, relative mobility on TLC and HPLC, and spectroscopic data.
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A novel tetracycline antibiotic, Sch 33256, was isolated from a culture broth of a new species of Actinomadura by solvent extraction, Sephadex G-25 column chromatography and crystallization and Spectroscopic analysis of the compound yielded 2'-N-methyl-8-methoxychlortetracy Cline as the proposed structure.
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