In vitro and in vivo antitumor effects of murine monoclonal antibody NCC-ST-421 reacting with dimeric Le(a) (Le(a)/Le(a)) epitope.

@article{Watanabe1991InVA,
  title={In vitro and in vivo antitumor effects of murine monoclonal antibody NCC-ST-421 reacting with dimeric Le(a) (Le(a)/Le(a)) epitope.},
  author={Masashi Watanabe and Tomokazu Ohishi and Masahiko Kuzuoka and E. D. Nudelman and Mark R. Stroud and Tetsuro Kubota and S Kodairo and Osahiko Abe and Setsuo Hirohashi and Yukio Shimosato},
  journal={Cancer research},
  year={1991},
  volume={51 8},
  pages={2199-204}
}
A murine monoclonal antibody (MAb), NCC-ST-421 (IgG3), was raised by using a human gastric cancer xenograft St-4 as immunogen. Immunization was achieved by transferring immunocompetent normal BALB/c mouse spleen cells into BALB/c-nu/nu mice bearing St-4 tumors. Hybridomas were produced from spleen cells of the mice after rejection of the tumors and were screened for preferential reactivity with cancers on formalin-fixed paraffin sections, as described previously for establishment of MAb NCC-ST… CONTINUE READING