In vitro and in silico analysis of the Aspergillus nidulans DNA-CreA repressor interactions.

Abstract

The CreA protein mediates carbon catabolite repression in the fungus Aspergillus nidulans. Its DNA-binding domain belongs to the Cys2-His2 class, binding specifically to a 5' SYGGRG 3' nucleotide sequence. EMSA experiments showed that the CreA(G27D) mutation resulted in a 30-fold increase of the Kdiss, and footprinting revealed a altered pattern of protein… (More)
DOI: 10.1080/07391102.2013.843474

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