In vitro analysis of type II polyketide synthase.

  title={In vitro analysis of type II polyketide synthase.},
  author={Wenjun Zhang and Yi Tang},
  journal={Methods in enzymology},
Bacterial type II polyketide synthases (PKSs) are responsible for the biosynthesis of therapeutically important aromatic polyketides, including daunorubicin and tetracycline. Discovery and cloning of the type II, actinorhodin biosynthetic gene cluster from Streptomyces coelicolor marked the beginning of a new era in natural product biosynthesis. While genetics has played an indispensable role in understanding the programming rules of this unique family of natural product biosynthetic pathways… CONTINUE READING