In vitro analysis of translational rate and accuracy with an unmodified tRNA.

@article{Harrington1993InVA,
  title={In vitro analysis of translational rate and accuracy with an unmodified tRNA.},
  author={K M Harrington and I A Nazarenko and Daniel B. Dix and Robert A. Thompson and O. C. Uhlenbeck},
  journal={Biochemistry},
  year={1993},
  volume={32 30},
  pages={7617-22}
}
Escherichia coli tRNA(Phe) transcript lacking all the modified nucleosides was investigated in an in vitro translation system. To estimate the affinity of tRNA toward EF-Tu, Kd and K-1 were measured by the nuclease protection assay, and it was shown that the absence of modifications decreases ternary complex stability less than 2-fold. The activity of unmodified Phe-tRNA(Phe) on E. coli ribosomes was compared to modified Phe-tRNA(Phe) using the framework of the kinetic proofreading mechanism… CONTINUE READING
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