In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional group I intron-encoded protein, I-AniI.

@article{Geese2003InVA,
  title={In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional group I intron-encoded protein, I-AniI.},
  author={William J. Geese and Yong Kuk Kwon and Xiaoping Wen and Richard B. Waring},
  journal={European journal of biochemistry},
  year={2003},
  volume={270 7},
  pages={
          1543-54
        }
}
The AnCOB group I intron from Aspergillus nidulans encodes a homing DNA endonuclease called I-AniI which also functions as a maturase, assisting in AnCOB intron RNA splicing. In this investigation we biochemically characterized the endonuclease activity of I-AniI in vitro and utilized competition assays to probe the relationship between the RNA- and DNA-binding sites. Despite functioning as an RNA maturase, I-AniI still retains several characteristic properties of homing endonucleases including… CONTINUE READING

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