In vitro analysis of human drug glucuronidation and prediction of in vivo metabolic clearance.

  title={In vitro analysis of human drug glucuronidation and prediction of in vivo metabolic clearance.},
  author={Matthew G. Soars and Brian Burchell and Robert J. Riley},
  journal={The Journal of pharmacology and experimental therapeutics},
  volume={301 1},
The glucuronidation of a number of commonly used hepatic uridine diphosphate glucuronosyltransferase drug substrates has been studied in human tissue microsomes. Prediction of in vivo hepatic drug glucuronidation from liver microsomal data yielded a consistent 10-fold under-prediction. Consideration of protein binding was observed to be pivotal when predicting in vivo glucuronidation for acid substrates. Studies using human intestinal microsomes demonstrated the majority of drugs to be… 

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