In vitro activity of a serine protease from Monacrosporium thaumasium fungus against first-stage larvae of Angiostrongylus vasorum

@article{Soares2011InVA,
  title={In vitro activity of a serine protease from Monacrosporium thaumasium fungus against first-stage larvae of Angiostrongylus vasorum},
  author={Filippe E Feitas Soares and F{\'a}bio Ribeiro Braga and Jackson Victor de Ara{\'u}jo and W. S. Lima and Lanuze R. Mozer and Jos{\'e} Humberto de Queiroz},
  journal={Parasitology Research},
  year={2011},
  volume={110},
  pages={2423-2427}
}
A serine protease from the nematophagous fungus Monacrosporium thaumasium (NF34a) was purified, partially characterized and tested in vitro in control of the first larval stage of Angiostrongylus vasorum. NF34a grew in liquid culture medium, producing its crude extract that was purified by ion exchange chromatography. The fractions with high protease activity were collected in a pool, and elution of proteases was monitored by enzymatic assay and protein content. Purification steps were… CONTINUE READING

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