In vitro activation of fish phagocytic cells by GH, prolactin and somatolactin.

@article{Sakai1996InVA,
  title={In vitro activation of fish phagocytic cells by GH, prolactin and somatolactin.},
  author={Masahiro Sakurai Toshiaki Sakai and Masashi Kobayashi and Hideyuki Kawauchi},
  journal={The Journal of endocrinology},
  year={1996},
  volume={151 1},
  pages={113-8}
}
The activation of rainbow trout (Oncorhyndus mykiss) phagocytic cells by chum salmon GH, prolactin (PRL) and somatolactin (SL) was investigated in vitro. Rainbow trout kidney leucocytes were cultured in RPMI 1640 medium containing 1, 10, 50 or 100 ng of each hormone/ml and the production of superoxide anion was measured using reduction of nitroblue tetrazolium (NBT) and ferricytochrome C. Macrophages incubated with 10-100 ng GH or PRL/ml showed significantly enhanced production of superoxide… CONTINUE READING

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