In vitro actions on hemopoietic cells of recombinant murine GM‐CSF purified after production in Escherichia coli: Comparison with purified native GM‐CSF

@article{Metcalf1986InVA,
  title={In vitro actions on hemopoietic cells of recombinant murine GM‐CSF purified after production in Escherichia coli: Comparison with purified native GM‐CSF},
  author={D. Metcalf and A. Burgess and G. Johnson and N. Nicola and E. Nice and J. Delamarter and D. Thatcher and J. Mermod},
  journal={Journal of Cellular Physiology},
  year={1986},
  volume={128}
}
Recombinant murine GM‐CSF produced in Escherichia coli was purified to homogeneity and tested in parallel with purified native GM‐CSF. Both recombinant and native GM‐CSF stimulated granulocyte and/or macrophage colony formation by adult and fetalmouse progenitor cells, and with adult marrow cells the specific activity of the recombinant GM‐CSF (25 × 108 U/mg) was similar to that of the native form (15 × 108 U/mg). At high concentrations (> 200 U/ml), both forms of GM‐CSF also stimulated… Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 27 REFERENCES
...
1
2
3
...