In vitro-Versuche mit Litomosoides carinii (Nematoda: Filarioidea)

  title={In vitro-Versuche mit Litomosoides carinii (Nematoda: Filarioidea)},
  author={P. Wenk and Brunhilde Illgen and H. M. Seitz},
  journal={Zeitschrift f{\"u}r Parasitenkunde},
SummaryEmbryos of L. carinii continue intrauterine development to microfilariae and are totally released into the medium within 5–6 days when the latter (Tc 199) is changed daily and air is used as the gas phase. Oogenesis or further fertilization of eggs, however, does not occur in vitro in any of the media examined by us. One female releases 140 × 103 microfilaria/day on an average in vitro within 5–6 days. Mean initial numbers of 300 × 103 Mf/♀/day are observed.Addition of equine serum… Expand
In vitro-Versuche mitLitomosoides carinii (Nematoda: Filarioidea)
When fetal calf serum or human serum is added to the culture medium Tc 199, adult females ofLitomosoides carinii release the same amount of microfilariae in vitro as after addition of cotton rat serum; moreover, addition ofMastomys serum immobilizes them. Expand
Fecundity ofLitomosoides carinii (Nematoda, Filarioidea) in vivo and in vitro
The course of embryogenesis from the fertilization of eggs to the delivery of the first microfilariae was observed by daily autopsies during prepatency and a positive correlation between the body length of a female worm and its number of embryos in utero was evident. Expand
Observations on adult Onchocerca volvulus maintained in vitro.
Adult Onchocerca volvulus were enzymatically isolated with collagenase from excised nodules and kept in TC medium 199 with Hank's salts supplemented with various sera, but the production of new oocytes or development of embryos could not be observed in vitro. Expand
Characterization of the exposed carbohydrates on the sheath surface of in vitro-derived Brugia pahangi microfilariae by analysis of lectin binding.
The results suggest that maturation and release of microfilariae are correlated with glycosidic alterations on the sheath surface, and in vitro-derived microFilariae could be differentiated by their characteristic of binding to a panel of fluorescein-conjugated lectins. Expand
Die Isolierung von Mikrofilarien vonLitomosoides carinii im Ficoll-Dichtegradienten
A method is described to isolate microfilariae of Litomosoides carinii on a Ficoll isopycnic gradient apparently without causing any alteration of the larval surface and vitality. Expand
Elimination of microfilariae (Litomosoides carinii Filarioidea) in the patent and in the immunized cotton-rat.
  • B. Haas, P. Wenk
  • Biology, Medicine
  • Transactions of the Royal Society of Tropical Medicine and Hygiene
  • 1981


Maintenance of filarial worms in vitro.
  • A. Taylor
  • Biology, Medicine
  • Experimental parasitology
  • 1960
Adult Litomosoides carinii and adult Dirofilaria immitis have been maintained in vitro for 23 days and the females have produced microfilariae for 18 days, and infected larvae of D. immitis remained alive and healthy for 9 days in vitro. Expand
The in vitro development of microfilariae of the dog heartworm Dirofilaria immitis to the "sausage-form".
The microfilariae of Dirofilaria immitis were cultured to the late first-stage (sausageform) at 27 C and at room temperature and comparable development was obtained in cell-free media. Expand
Dipetalonema viteae in the experimentally infected jird, Meriones unguiculatus. I. Insemination, development from egg to microfilaria, reinsemination, and longevity of mated and unmated worms.
Dipetalonema viteae in Meriones unguiculatus usually completed the 3rd molt at 7 days and the 4th moltAt 23 days of infection, insemination occurred most frequently at 25 to 28 days, indicating that for continuous fertility when few worms are present frequent mating is required. Expand
Development in vitro of microfilariae of Wuchereria bancrofti and Litomosoides carinii as rar as the sausage stage
  • Ann . roy . Soc . trop . Med .
  • 1975
" Populationsdynamiche Untersuchungen an Litomosoides carinii ( Nematoda , Filariidae )
  • Ann . trop . Medl Parasit .
  • 1972
Klinische Chemie und Mikroskopie
Die Regulation der Parasitämie bei Filarien ( Litomosoides carinii , Nematoda , Filariidae )
  • Zool . Anz .
  • 1970