In vitro-Versuche mit Litomosoides carinii (Nematoda: Filarioidea)

@article{Wenk2004InVM,
  title={In vitro-Versuche mit Litomosoides carinii (Nematoda: Filarioidea)},
  author={P. Wenk and Brunhilde Illgen and H. M. Seitz},
  journal={Zeitschrift f{\"u}r Parasitenkunde},
  year={2004},
  volume={55},
  pages={63-72}
}
SummaryEmbryos of L. carinii continue intrauterine development to microfilariae and are totally released into the medium within 5–6 days when the latter (Tc 199) is changed daily and air is used as the gas phase. Oogenesis or further fertilization of eggs, however, does not occur in vitro in any of the media examined by us. One female releases 140 × 103 microfilaria/day on an average in vitro within 5–6 days. Mean initial numbers of 300 × 103 Mf/♀/day are observed.Addition of equine serum… Expand
In vitro-Versuche mitLitomosoides carinii (Nematoda: Filarioidea)
TLDR
When fetal calf serum or human serum is added to the culture medium Tc 199, adult females ofLitomosoides carinii release the same amount of microfilariae in vitro as after addition of cotton rat serum; moreover, addition ofMastomys serum immobilizes them. Expand
Fecundity ofLitomosoides carinii (Nematoda, Filarioidea) in vivo and in vitro
TLDR
The course of embryogenesis from the fertilization of eggs to the delivery of the first microfilariae was observed by daily autopsies during prepatency and a positive correlation between the body length of a female worm and its number of embryos in utero was evident. Expand
Observations on adult Onchocerca volvulus maintained in vitro.
TLDR
Adult Onchocerca volvulus were enzymatically isolated with collagenase from excised nodules and kept in TC medium 199 with Hank's salts supplemented with various sera, but the production of new oocytes or development of embryos could not be observed in vitro. Expand
Characterization of the exposed carbohydrates on the sheath surface of in vitro-derived Brugia pahangi microfilariae by analysis of lectin binding.
TLDR
The results suggest that maturation and release of microfilariae are correlated with glycosidic alterations on the sheath surface, and in vitro-derived microFilariae could be differentiated by their characteristic of binding to a panel of fluorescein-conjugated lectins. Expand
Die Isolierung von Mikrofilarien vonLitomosoides carinii im Ficoll-Dichtegradienten
TLDR
A method is described to isolate microfilariae of Litomosoides carinii on a Ficoll isopycnic gradient apparently without causing any alteration of the larval surface and vitality. Expand
Elimination of microfilariae (Litomosoides carinii Filarioidea) in the patent and in the immunized cotton-rat.
  • B. Haas, P. Wenk
  • Biology, Medicine
  • Transactions of the Royal Society of Tropical Medicine and Hygiene
  • 1981

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