In vitro Excystation of Metacercariae of Clinostomum complanatum (Rudolphi, 1819) (Digenea: Clinostomatidae)

  title={In vitro Excystation of Metacercariae of Clinostomum complanatum (Rudolphi, 1819) (Digenea: Clinostomatidae)},
  author={A. M. N. Kalantan and Misbahul Arfin},
  journal={Acta Zoologica},
In vitro excystation of metacercariae of Clinostomum complanatum (Rudolphi, 1819) was studied. Excystation was an active process, initiated by a rise in temperature. The metacercariae excysted in enzymic as well as non enzymic medium, but the rate of excystation was affected by the temperature and/or pH of the medium. The optimal excystation was obtained in pepsin-Tyrode at pH 2.3-3.5 and a temperature of 42°C. Trypsin treatment was not required. 
3 Citations
Infection of Clinostomum complanatum (Treamdoda: Digenea) metacercaria in cultured southern top mouthed minnow Pseudorasbora parva
Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.
The effects of age, sex and breed of chicken on susceptibility to Clinostomum complanatum (Digenea: Clinostomatidae)
Mature male chickens were more susceptible than the females and the single comb white Leghorn was found to be susceptible while the local type and the cross breed were not susceptible to C. complanatum.
Metacercarial excystment of trematodes.
  • B. Fried
  • Biology
    Advances in parasitology
  • 1994


Development of Microphalloides japonicus (Osborn, 1919) metacercariae in vitro (Trematoda: Microphallidae)
ABSTRACT Metacercariae of the trematode Microphalloides japonicus (Osborn, 1919) were cultivated in various media to gravid adults, and the results were compared with those in vivo. A few eggs were
Excystation of metacercariae of Posthodiplostomum minimum minimum Hoffman, 1958, and their development in the chick and on the chorioallantois.
  • B. Fried
  • Biology
    The Journal of parasitology
  • 1970
Sexual maturation of trematode metacercariae of Posthodiplostomum minimum minimum from cyprinid fishes occurred within 27 hr in the domestic chick and on the chorioallantois.
Autoradiographic studies on the reproductive system of Philophthalmus hegeneri Penner and Fried, 1963.
Timing of stages of gametogenesis and migration of vitelline cells as well as patterns of sperm transfer in single and multiple infections were determined in this study.
A Study of some of the Factors Affecting Excystation in vitro of the Metacercarial Stages of Holostephanus lühei Szidat, 1936 and Cyathocotyle bushiensis Khan, 1962 (Strigeida: Trematoda)
In many digenetic trematode life-cycles there occurs a metacercarial stage which is usually the ultimate infective stage for the final host. The metacercarial stage may be free and active as in