In vitro DNA binding of the archaeal protein Sso7d induces negative supercoiling at temperatures typical for thermophilic growth.

@article{LpezGarca1998InVD,
  title={In vitro DNA binding of the archaeal protein Sso7d induces negative supercoiling at temperatures typical for thermophilic growth.},
  author={Purificaci{\'o}n L{\'o}pez-Garc{\'i}a and Stefan Knapp and Rudolf Ladenstein and Patrick Forterre},
  journal={Nucleic acids research},
  year={1998},
  volume={26 10},
  pages={2322-8}
}
The topological state of DNA in hyperthermophilic archaea appears to correspond to a linking excess in comparison with DNA in mesophilic organisms. Since DNA binding proteins often contribute to the control of DNA topology by affecting DNA geometry in the presence of DNA topoisomerases, we tested whether the histone-like protein Sso7d from the hyperthermophilic archaeon Sulfolobus solfataricus alters DNA conformation. In ligase-mediated supercoiling assays carried out at 37, 60, 70, 80 and 90… CONTINUE READING
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