In vitro Augmentation of Natural Killer Cell Activity and Production of Interferon‐α/β and ‐γ with Deoxyribonucleic Acid Fraction from Mycobacterium bovis BCG

@article{Yamamoto1988InVA,
  title={In vitro Augmentation of Natural Killer Cell Activity and Production of Interferon‐$\alpha$/$\beta$ and ‐$\gamma$ with Deoxyribonucleic Acid Fraction from Mycobacterium bovis BCG},
  author={Saburo Yamamoto and Etsuro Kuramoto and Shizuo Shimada and Tohru Tokunaga},
  journal={Japanese Journal of Cancer Research : Gann},
  year={1988},
  volume={79},
  pages={866 - 873}
}
A nucleic acid‐rich fraction extracted and purified from BCG (MY‐1) augmented natural killer (NK) cell activity of mouse spleen cells in vitro, and produced factor(s) which showed anti‐viral activity and rendered normal macrophages cytotoxic towards tumor cells. These cellular responses were induced by the MY‐1 digested preliminarily with RNase, but not by the MY‐1 digested with DNase, indicating that DNA contained in MY‐1 was essential for the responses. The function of the factor to activate… 
A SYNTHETIC SINGLE‐STRANDED DNA, POLY(dG, dC), INDUCES TNTERFERON‐α/β AND ‐γ, AUGMENTS NATURAL KILLER ACTIVITY, AND SUPPRESSES TUMOR GROWTH
TLDR
Direct cytotoxicity of poly(dG, dC) at a concentration of 1,000 μg/ml against IMC cells was not observed in vitro, and the virus‐inhibitory activity of the supernatant was mostly neutralized by anti‐IFNα/β.
Activation of NK Cell By Immunostimulatory Oligo-DNA in Mouse and Human
It was reported that a purified DNA-rich fraction, MY-1, extracted from Mycobacterium bovis Bacille Calmette Guerin BCG, exhibits a strong antitumor activity against various syngeneic mouse and
In Situ Infiltration of Natural Killer‐Like Cells Induced by Intradermal Injection of the Nucleic Acid Fraction from BCG
TLDR
Results indicate that the nucleic acid components of MY‐1 are responsible for the induced in situ infiltration of mononuclear cells that markedly resembled natural killer (NK) cells in their cytochemical characteristics and surface markers.
In vitro augmentation of macrophage-activating-factor release from peripheral blood cells of cancer patients by a DNA fraction from Mycobacterium bovis BCG.
TLDR
It is suggested that a DNA-rich fraction from Mycobacterium bovis BCG (MY-1) augments MAF-activity release from PBL by inducing IFN-gamma and other M AF-like substances.
Activation of NK cell (human and mouse) by immunostimulatory DNA sequence
TLDR
Antitumor activity of MY-1 was also abolished if the animals were pretreated with asialo GM1 antiserum or carrageenan suggesting that the activity can be ascribed mainly to activated NK cells [16].
Synthetic Oligonucleotides with Particular Base Sequences from the cDNA Encoding Proteins of Mycobacterium bovis BCG Induce Interferons and Activate Natural Killer Cells
TLDR
It is suggested that certain palindrome sequences, like GACGTC, GGCGCC and TGCGCA, are essential for 30‐mer oligonucleotides, like BCG‐A4a, to induce IFNs.
Resistance to Pseudorabies Virus with Enhanced Interferon Production and Natural Killer Cell Activity in Mice Treated with Serum Thymic Factor
TLDR
Interferon production and NK cell activity were enhanced in the FTS‐pretreated mice, suggesting that interferon may play an important role in this F TS‐induced resistance to PRV infection.
CpG oligodeoxynucleotides promote the host protective response against infection with Cryptococcus neoformans through induction of interferon‐gamma production by CD4+ T cells
TLDR
CpG‐ODN alters the Th1–Th2 cytokine balance and promotes host resistance against infection with C. neoformans, and the protective effect of this agent was strongly inhibited by neutralizing anti‐TNF‐α MoAb.
DNA FROM MYCOBACTERIUM BOVIS (BCG) AND OLIGONUCLEOTIDES SUPPRESSED IGE PRODUCTION BY HUMAN LYMPHOCYTES: PRELIMINARY STUDY
TLDR
Results suggest that exposure to MY-1 or the oligonucleotides including the CGTTCG sequence may be a novel strategy for the treatment of IgE-related allergic diseases.
DNA activates human immune cells through a CpG sequence‐dependent manner
TLDR
It is demonstrated that bacterial DNA and CpG ODN induce proliferation of B cells, while other subpopulations, such as monocytes and T cells, did not proliferate and it is concluded that monocytes, but not B or T’cells, represent the prime source of cytokines.
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References

SHOWING 1-10 OF 12 REFERENCES
In vivo augmentation of natural killer cell activity with a deoxyribonucleic acid fraction of BCG.
TLDR
Antitumor activities of MY-1 were also abolished if the animals were pretreated with anti-asialo GM1 antiserum or carrageenan, suggesting that the activities can be ascribed mainly to activated NK cells.
Natural cell‐mediated cytotoxicity in rats. III. Effects of immunopharmacologic treatments on natural reactivity and on reactivity augmented by polyinosinic‐polycytidylic acid
TLDR
It is suggested that phagocytes may play a role in maintaining high levels of NK activity in vivo and, further, may be involved in the mechanism by which natural cytotoxicity is boosted by poly I:C.
Natural cell‐mediated cytotoxicity in rats. II. In vivo augmentation of NK‐cell activity
TLDR
Data indicate that interferon may play a central role in the augmentation of NK activity in vivo, with a shorter time course of augmented activity seen after inoculation with poly I:C.
Antitumor activity of deoxyribonucleic acid fraction from Mycobacterium bovis BCG. I. Isolation, physicochemical characterization, and antitumor activity.
TLDR
A fraction extracted from Mycobacterium bovis strain BCG, which was composed of 70% DNA, 28.0% RNA, 1.3% protein, 0.20% glucose, and 0.1% lipid, was found to possess strong antitumor activity, suggesting that the DNA from BCG possessed strong antitUMor activity under certain conditions.
Antitumor activity of the DNA fraction from Mycobacterium bovis BCG. II. Effects on various syngeneic mouse tumors.
TLDR
MY-1 was equally effective in mice with or without presensitization with BCG, whereas BCG was much more effective in BCG-sensitized mice, suggesting that a delayed-type hypersensitivity reaction elicited by BCG protein is not required for the antitumor activity of MY-1.
Induction of interferon synthesis by synthetic double-stranded polynucleotides
TLDR
Whether a double-stranded deoxyribonucleic acid (DNA) polymer formed frompolydeoxyinosinic acid (dl) and polydeoxycytidylic acid (dC), as well as a double thestranded RNA-DNA homopolymer hybrid formed with rI and dC, could also induce interference and interferon synthesis in a cell culture is found.
Inducers of interferon and host resistance. I. Double-stranded RNA from extracts of Penicillium funiculosum.
TLDR
The present report describes the isolation and characterization of a double-stranded RNA from extracts of Penicillium funiculosum (helenine) which, when freed of protein, is a highly active inducer of interferon and host resistance.
Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes.
TLDR
The discovery that certain multistranded polynucleotide complexes are highly active in microgram amounts in inducing interferon and host resistance in vivo arid in vitro is reported.
The specificity of interferon induction in chick embryo cells by helical RNA.
  • C. Colby, M. Chamberlin
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1969
TLDR
The high degree of specificity of the induction process is consistent with the existence of a specific intracellular receptor site, which may be a protein.
STUDIES ON INTERFERON.
  • A. Isaacs
  • Biology
    The Australian journal of experimental biology and medical science
  • 1965
TLDR
Since new evidence has been produced that it is a normal cellular product whose synthesis is set off by many different stimuli, there has been some speculation about its role in nonnal cells.
...
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