In vitro 32P-labelling of viroid RNA for hybridization studies.

Abstract

A method is described for the in vitro labelling of viroid RNA for use in hybridization studies. The citrus exocortis viroid (approximately 350 nucleotides) is degraded by hot formamide hydrolysis to fragments ranging from small oligonucleotides to near full lengths, and subsequently labelled to high specific activity by enzymatically attaching 32P to the 5… (More)

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@article{Negruk1980InV3, title={In vitro 32P-labelling of viroid RNA for hybridization studies.}, author={Valentin I. Negruk and Ludwig Grill and Joseph S. Semancik}, journal={Journal of virological methods}, year={1980}, volume={1 4}, pages={229-34} }