In vitro, long-range sequence information for de novo genome assembly via transposase contiguity.

@article{Adey2014InVL,
  title={In vitro, long-range sequence information for de novo genome assembly via transposase contiguity.},
  author={Andrew C Adey and Jacob O. Kitzman and Joshua N. Burton and Riza M Daza and Akash Kumar and Lena Christiansen and Mostafa Ronaghi and Sasan Amini and Kevin L. Gunderson and Frank Steemers and Jay Shendure},
  journal={Genome research},
  year={2014},
  volume={24 12},
  pages={2041-9}
}
We describe a method that exploits contiguity preserving transposase sequencing (CPT-seq) to facilitate the scaffolding of de novo genome assemblies. CPT-seq is an entirely in vitro means of generating libraries comprised of 9216 indexed pools, each of which contains thousands of sparsely sequenced long fragments ranging from 5 kilobases to > 1 megabase. These pools are "subhaploid," in that the lengths of fragments contained in each pool sums to ∼5% to 10% of the full genome. The scaffolding… CONTINUE READING
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