In utero intraventricular injection and electroporation of E15 mouse embryos.

@article{Walantus2007InUI,
  title={In utero intraventricular injection and electroporation of E15 mouse embryos.},
  author={William Walantus and David Casta{\~n}eda and Laura El{\'i}as and Arnold R Kriegstein},
  journal={Journal of visualized experiments : JoVE},
  year={2007},
  volume={6},
  pages={239}
}
In-utero in-vivo injection and electroporation of the embryonic mouse neocortex provides a powerful tool for the manipulation of individual progenitors lining the walls of the lateral ventricle. This technique is now widely used to study the processes involved in corticogenesis by over-expressing or knocking down genes and observing the effects on cellular proliferation, migration, and differentiation. In comparison to traditional knockout strategies, in-utero electroporation provides a rapid… CONTINUE READING
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