In utero gene therapy: transfer and long-term expression of the bacterial neo(r) gene in sheep after direct injection of retroviral vectors into preimmune fetuses.

@article{Porada1998InUG,
  title={In utero gene therapy: transfer and long-term expression of the bacterial neo(r) gene in sheep after direct injection of retroviral vectors into preimmune fetuses.},
  author={Christopher D. Porada and Nam D. Tran and M A Eglitis and Robert C. Moen and L Troutman and Alan W. Flake and Yongjun Zhao and W. French Anderson and Esmail D. Zanjani},
  journal={Human gene therapy},
  year={1998},
  volume={9 11},
  pages={1571-85}
}
We investigated whether directly injecting retroviral vectors into preimmune fetuses could result in the transfer and long-term expression of exogenous genes. Twenty-nine preimmune sheep fetuses were injected with helper-free retroviral vector preparations. Twenty-two fetuses survived to term, 4 of which were sacrificed at birth. Of the remaining 18 animals, 3 were controls and 15 had received vector preparations. Twelve of these 15 animals demonstrated transduction of hematopoietic cells when… CONTINUE READING

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