In utero delivery of adeno-associated viral vectors: intraperitoneal gene transfer produces long-term expression.

@article{Lipshutz2001InUD,
  title={In utero delivery of adeno-associated viral vectors: intraperitoneal gene transfer produces long-term expression.},
  author={Gerald S. Lipshutz and Carol A Gruber and Y Cao and John Hardy and Christopher H. Contag and Karin M. L. Gaensler},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2001},
  volume={3 3},
  pages={284-92}
}
Recombinant adeno-associated viruses (rAAV) are promising gene transfer vectors that produce long-term expression without toxicity. To investigate future approaches for in utero gene delivery, the efficacy and safety of prenatal administration of rAAV were determined. Using luciferase as a reporter, expression was assessed by whole-body imaging and by analysis of luciferase activity in tissue extracts, at the time of birth and monthly thereafter. Transgene expression was detected in all… CONTINUE READING