In-straw cryoprotectant dilution of IVP bovine blastocysts vitrified in hand-pulled glass micropipettes.

@article{Vieira2007InstrawCD,
  title={In-straw cryoprotectant dilution of IVP bovine blastocysts vitrified in hand-pulled glass micropipettes.},
  author={Adriane Vieira and Fabiana Forell and Cristiano Feltrin and Jos{\'e} G. L. Rodrigues},
  journal={Animal reproduction science},
  year={2007},
  volume={99 3-4},
  pages={377-83}
}
The aim of this study was to determine the influence of two ethylene glycol-based vitrification solutions on in vitro and in vivo survival after in-straw cryoprotectant dilution of vitrified in vitro-produced bovine embryos. Day-7 expanded blastocysts were selected according to diameter (> or = 180 microm) and osmotic characteristics and randomly assigned to one of three groups (i) VSa: vitrification in 40% EG+17.1% SUC+0.1% PVA; (ii) VSb: vitrification in 20% EG+20% DMSO; (iii) control: non… CONTINUE READING