In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopy.

@article{Burghardt2006InSS,
  title={In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopy.},
  author={Thomas P Burghardt and Katalin Ajtai and Julian Borejdo},
  journal={Biochemistry},
  year={2006},
  volume={45 13},
  pages={4058-68}
}
Confocal microscopy is widely used for acquiring high spatial resolution tissue sample images of interesting fluorescent molecules inside cells. The fluorescent molecules are often tagged proteins participating in a biological function. The high spatial resolution of confocal microscopy compared to wide field imaging comes from an ability to optically isolate and image exceedingly small volume elements made up of the lateral (focal plane) and depth dimensions. Confocal microscopy at the optical… CONTINUE READING

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