In-situ protein determination to monitor contamination in a centrifugal partition chromatograph.

@article{Bouiche2017InsituPD,
  title={In-situ protein determination to monitor contamination in a centrifugal partition chromatograph.},
  author={Feriel Bouiche and Karine Faure},
  journal={Analytical biochemistry},
  year={2017},
  volume={525},
  pages={
          23-28
        }
}
Centrifugal partition chromatography (CPC) works with biphasic liquid systems including aqueous two-phase systems. Metallic rotors are able to retain an aqueous stationary phase able to purify proteins. But the adhesion of proteins to solid surface may pose a cross-contamination risk during downstream processes. So it is of utmost importance to ensure the cleanliness of the equipment and detect possible protein contamination in a timely manner. Thereby, a direct method that allows the… CONTINUE READING
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