In situ probing of gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides.

@article{Roller1995InSP,
  title={In situ probing of gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides.},
  author={C. Roller and Michael Wagner and Rudolf I. Amann and Wolfgang Ludwig and Karl Heinz Schleifer},
  journal={Microbiology},
  year={1995},
  volume={141 ( Pt 5)},
  pages={
          1267
        }
}
Lehrstuhl fur Mikrobiologie, Tec hn isc he U n ive rsitat Munchen, ArcisstraBe 16, D80290 Mu nchen, Germany 235-rRNA-targeted oligonucleotide probes were designed for the phylogenetic group Gram-positive bacteria with high G + C content of DNA ' (GPBHGC). A sequence idiosyncrasy in two adjacent base pairs in the stem of helix 69 in domain IV of the 235 rRNA is present in all hitherto analysed strains of GPBHGC. An oligonucleotide probe targeted to this region hybridized only with strains of… 
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