In situ kinetic analysis of glyoxalase I and glyoxalase II in Saccharomyces cerevisiae.

@article{Martins2001InSK,
  title={In situ kinetic analysis of glyoxalase I and glyoxalase II in Saccharomyces cerevisiae.},
  author={Alice Maria Costa Martins and Pedro M. Mendes and Carlos Cordeiro and Ana Ponces Freire},
  journal={European journal of biochemistry},
  year={2001},
  volume={268 14},
  pages={3930-6}
}
The kinetics of glyoxalase I [(R)-S-lactoylglutathione methylglyoxal-lyase; EC 4.4.1.5] and glyoxalase II (S-2-hydroxyacylglutathione hydrolase; EC 3.1.2.6) from Saccharomyces cerevisiae was studied in situ, in digitonin permeabilized cells, using two different approaches: initial rate analysis and progress curves analysis. Initial rate analysis was performed by hyperbolic regression of initial rates using the program HYPERFIT. Glyoxalase I exhibited saturation kinetics on 0.05-2.5 mM… CONTINUE READING