In situ hybridization with nonisotopic probes using different detection systems.

@article{Holm1992InSH,
  title={In situ hybridization with nonisotopic probes using different detection systems.},
  author={Ruth Holm and Frank Karlsen and Jahn Marthin Nesland},
  journal={Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc},
  year={1992},
  volume={5 3},
  pages={315-9}
}
In order to evaluate a sensitive nonisotopic in situ hybridization method for routine work in pathology laboratories, we compared seven different detection systems, using digoxigenin- and biotin-labeled probes. The sensitivity of these methods was tested on four cases of cervical condyloma all known to be positive for HPV 6. Four of these methods gave satisfactory results without any background staining. The single biotin method and the single digoxigenin method were equally sensitive, while… CONTINUE READING