In situ hybridization using PEG-embedded tissue and riboprobes: increased cellular detail coupled with high sensitivity.

@article{Clayton1989InSH,
  title={In situ hybridization using PEG-embedded tissue and riboprobes: increased cellular detail coupled with high sensitivity.},
  author={David F. Clayton and Arturo Alvarez-Buylla},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1989},
  volume={37 3},
  pages={389-93}
}
We describe a procedure for preparing tissue sections by embedding in polyethylene glycol for subsequent in situ hybridization analysis using single-stranded RNA probes. Improved tissue morphology is obtained as compared to frozen sections, and the embedding procedure is milder and faster than paraffin embedding. Sections as thin as 2 microns are readily cut from PEG-embedded brain tissue. A simplified hybridization protocol (Clayton et al.: Neuron 1:249, 1988) supports the detection of even… CONTINUE READING
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