In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea

@inproceedings{King2013InSH,
  title={In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea},
  author={Ryan S. King and Phillip A Newmark},
  booktitle={BMC Developmental Biology},
  year={2013}
}
The freshwater planarian Schmidtea mediterranea has emerged as a powerful model for studies of regenerative, stem cell, and germ cell biology. Whole-mount in situ hybridization (WISH) and whole-mount fluorescent in situ hybridization (FISH) are critical methods for determining gene expression patterns in planarians. While expression patterns for a number of genes have been elucidated using established protocols, determining the expression patterns for particularly low-abundance transcripts… CONTINUE READING