In situ hybridization in wholemounted lamprey spinal cord: localization of netrin mRNA expression.

@article{Shifman2000InSH,
  title={In situ hybridization in wholemounted lamprey spinal cord: localization of netrin mRNA expression.},
  author={Michael I Shifman and Michael E Selzer},
  journal={Journal of neuroscience methods},
  year={2000},
  volume={104 1},
  pages={19-25}
}
The lamprey has been used as a model for the study of vertebrate neuronal circuitry and spinal cord regeneration. One of the advantages of this preparation is the ability to view the entire CNS in wholemount, including several identified neurons and neuron groups. However, because of difficulties in penetration of molecular reagents past the dense meninx primitiva and glia limitans, there has been no reliable method for in situ hybridization in spinal cord wholemounts. We now describe a… CONTINUE READING