In situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA.

@article{Thiry1989InSH,
  title={In situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA.},
  author={Marc Thiry and L. Thiry-Blaise},
  journal={European journal of cell biology},
  year={1989},
  volume={50 1},
  pages={235-43}
}
In situ hybridization using biotinylated rDNA probes and secondary antibody coupled to gold particles was developed on ultrathin sections of Lowicryl-embedded Ehrlich tumor cells for precise localization of ribosomal RNA (rRNA) and ribosomal DNA (rDNA). For the detection of rDNA, an immunocytochemical approach involving an antibody against single-stranded… CONTINUE READING