In situ hybridization as a technique for the immunological investigation of canine intestine: jejunal expression of IFNgamma and IL10 in Irish setters and beagles.

@article{Garden1999InSH,
  title={In situ hybridization as a technique for the immunological investigation of canine intestine: jejunal expression of IFNgamma and IL10 in Irish setters and beagles.},
  author={O. Garden and C. Elwood and M. Desport and R. Batt},
  journal={Veterinary immunology and immunopathology},
  year={1999},
  volume={70 1-2},
  pages={
          1-17
        }
}
In situ hybridization (ISH) has found numerous applications in biology and medicine. However, its use to demonstrate expression of cytokines within the canine small intestine has not been described. Digoxigenin-labelled riboprobes complementary to mRNA encoding canine IFNgamma and IL10 were used to demonstrate expression of these cytokines within formalin-fixed, paraffin-embedded sections of jejunum obtained from healthy control Irish setter (IS) dogs (n = 4), gluten-sensitive IS in remission… Expand
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Chapter 3 – Gastrointestinal Immunology

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