In situ hybridization as a technique for the immunological investigation of canine intestine: jejunal expression of IFNgamma and IL10 in Irish setters and beagles.

  title={In situ hybridization as a technique for the immunological investigation of canine intestine: jejunal expression of IFNgamma and IL10 in Irish setters and beagles.},
  author={O. Garden and C. Elwood and M. Desport and R. Batt},
  journal={Veterinary immunology and immunopathology},
  volume={70 1-2},
In situ hybridization (ISH) has found numerous applications in biology and medicine. However, its use to demonstrate expression of cytokines within the canine small intestine has not been described. Digoxigenin-labelled riboprobes complementary to mRNA encoding canine IFNgamma and IL10 were used to demonstrate expression of these cytokines within formalin-fixed, paraffin-embedded sections of jejunum obtained from healthy control Irish setter (IS) dogs (n = 4), gluten-sensitive IS in remission… Expand
Upregulation of toll-like receptors in chronic enteropathies in dogs.
Bacteria-responsive TLR2, 4, and 9 are upregulated in duodenal and colonic mucosa in IBD, which might lead to increased inflammation through interaction with the commensal flora. Expand
Quantitative analysis of inflammatory and immune responses in dogs with gastritis and their relationship to Helicobacter spp. infection.
It is concluded that lymphoplasmacytic gastritis in dogs is characterized by concurrent activation of proinflammatory and immunomodulatory cytokines, with increased mRNA expression related to mucosal pathology. Expand
Cytokine mRNA abundance in intestinal biopsies from dogs with chronic diarrhea
It is postulated that patterns of selected key cytokines would help to objectively differentiate between the two subcategories of chronic enteropathies in dogs, and mRNA patterns ofselected cytokines in dogs with chronic enteropathy were studied. Expand
Distribution pattern of bovine viral diarrhoea virus type 1 genome in lymphoid tissues of experimentally infected sheep
The results show that the kinetics of BVDV-1 distribution in lymphoid tissues of experimentally infected non-pregnant sheep follows almost a similar pattern to that demonstrated in BVDVs infected cattle. Expand
A comprehensive pathological survey of duodenal biopsies from dogs with diet-responsive chronic enteropathy.
In dogs with diet-responsive CE, the remission of clinical signs with dietary therapy is associated with subtle decreases in lamina propria density of eosinophils and mononuclear cells, and resolution of ultrastructural lesions of the enterocyte. Expand
Chronic intestinal inflammation and intestinal disease in dogs.
The factors involved in breakdown of mucosal tolerance, the immunologic basis of canine enteropathies, and the use of novel immunotherapies for these diseases are discussed. Expand
Les Maladies Inflammatoires Chroniques Intestinales (MICI) des carnivores domestiques sont des affections intestinales chroniques se caractérisant par une infiltration de la lamina propria deExpand
Chapter 3 – Gastrointestinal Immunology


Expression of tumour necrosis factor-alpha, interleukin-6, and interleukin-2 mRNA in the jejunum of patients with coeliac disease.
This study shows that the immune response to gliadin appears to occur in the lamina propria and supports cell-mediated immunity in the pathogenesis of coeliac disease. Expand
Nested Polymerase Chain Reaction and In Situ Hybridization for Diagnosis of Canine Herpesvirus Infection in Puppies
ISH and PCR are both useful methods for diagnosing CHV infection in formalin-fixed, paraffin-embedded tissues and are highly specific and sensitive methods for further investigations of the pathogenesis of CHV-induced lesions. Expand
In vivo and in vitro detection of canine distemper virus nucleoprotein gene with digoxigenin-labelled RNA, double-stranded DNA probes and oligonucleotides by in situ hybridization.
RNA probes are currently the most sensitive tool for detection of CDV RNA in tissues, indicating that length of the probe is an important parameter when selecting probes. Expand
Cytokine mRNA expression in the mucosa of treated coeliac patients after wheat peptide challenge.
Findings suggest that the histological changes seen previously in the mucosa of coeliac patients after wheat peptide challenge may be caused by increased expression of cytokines within the mucosal. Expand
Detection of Canine Parvovirus in Naturally Infected Dogs with Enteritis and Myocarditis by in Situ Hybridization
  • W. Nho, J. Sur, A. Doster, S. B. Kim
  • Biology, Medicine
  • Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 1997
An improved method for the diagnosis of canine parvovirus using in situ hybridization in standard formalin-fixed, paraffin-embedded tissue sections was developed and suggests a broadened tissue tropism not seen before in Korean strains of canineParvov virus. Expand
Specific diagnosis of parvovirus enteritis in dogs and cats by in situ hybridization.
It is concluded that an aetiological diagnosis can be established with a high degree of certainty by routine histology and demonstration of the infectious agent by in situ hybridization proves to be a valuable specific tool which allows an exact cellular localization of parvovirus in formalin-fixed, paraffin wax-embedded tissue sections. Expand
Detection of bovine virus diarrhoea virus RNA by in situ hybridisation with digoxigenin-labelled riboprobes.
In situ hybridisation has facilitated a precise examination, at the cellular level, of viral nucleic acid distribution during pestiviral infections, with an increased sensitivity and wider applicability than immunocytochemistry. Expand
Diagnosis of Aujeszky's disease virus infection in dogs by use of immunohistochemistry and in-situ hybridization.
Both immunohistochemistry and in-situ hybridization techniques are valid techniques to confirm ADV infection in paraffin-embedded tissues and will be useful for characterizing the pathogenesis of ADV in the central nervous system (CNS) in carnivores. Expand
Analysis of interleukin-4 and interleukin-10 and their association with the lymphocytic infiltrate in the small intestine of patients with coeliac disease.
Investigating whether there is a primary deficiency of IL-4 and IL-10 producing cells and their site of production in the small intestine of patients with coeliac disease found no primary deficiency and it is suggested that these cytokines are involved in normal mucosal immunoregulation. Expand
T cells of the human intestinal lamina propria are high producers of interleukin-10
Background and aim —Some of the recently observed functional features characteristic of immunocompetent cells residing in the human intestinal lamina propria could be mediated by interleukin- 10Expand