In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP).

@article{Quinn2015InSD,
  title={In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP).},
  author={Jeffrey J. Quinn and Howard Y Chang},
  journal={Methods in molecular biology},
  year={2015},
  volume={1262},
  pages={199-213}
}
Here we describe domain-specific chromatin isolation by RNA purification (dChIRP), a technique for dissecting the functional domains of a target RNA in situ. For an RNA of interest, dChIRP can identify domain-level intramolecular and intermolecular RNA-RNA, RNA-protein, and RNA-DNA interactions and maps the RNA's genomic binding sites with higher precision than domain-agnostic methods. We illustrate how this technique has been applied to the roX1 lncRNA to resolve its domain-level architecture… CONTINUE READING
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