"In situ" determinations of apparent Km and Vmax of brush border disaccharidases along the villi of normal human jejunal biopsy specimens. A quantitative histochemical study.

Abstract

In order to characterize lactase/beta-glucosidase and the neutral alpha-glucosidase kinetically in the brush border membrane at different villus sites of normal human jejunal mucosa, a quantitative histochemical study has been carried out on biopsy specimens of patients only suffering from functional disturbances revealing normal mucosal architecture. The apparent Vmax-values of both alpha- and beta-glucosidases increased significantly from the villus base to the transition zone between medium and apical villus third. During enterocyte maturation on the villus no changes in the apparent Km-values could be detected. Equally the ratios between the Vmax-values of the disaccharidases remained constant. Male persons exhibited significantly greater apparent Vmax-values for alpha-glucosidase than females at both villus measuring positions. The findings can be taken as a starting point for the evaluation of local changes of apparent enzyme-kinetic data under pathological conditions.

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@article{Gutschmidt1981InSD, title={"In situ" determinations of apparent Km and Vmax of brush border disaccharidases along the villi of normal human jejunal biopsy specimens. A quantitative histochemical study.}, author={Sven Gutschmidt}, journal={Histochemistry}, year={1981}, volume={71 3}, pages={451-62} }