In situ determination of [Ca2+]i threshold for translocation of the alpha-protein kinase C isoform.

@article{Khalil1994InSD,
  title={In situ determination of [Ca2+]i threshold for translocation of the alpha-protein kinase C isoform.},
  author={Raouf Ali Khalil and Claude Lajoie and Kathleen G Morgan},
  journal={The American journal of physiology},
  year={1994},
  volume={266 6 Pt 1},
  pages={C1544-51}
}
Because of inherent difficulties in maintaining physiological conditions in biochemical assays, the intracellular free Ca2+ concentration ([Ca2+]i) required for activation of protein kinase C (PKC) in intact cells remains unclear. In the present study, [Ca2+]i was measured in freshly isolated vascular smooth muscle cells loaded with fura 2 while, in parallel, the distribution of the Ca(2+)-dependent alpha-PKC isoform was monitored using digital imaging microscopy. The [Ca2+]i alpha-PKC… CONTINUE READING
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