In situ detection of transrenal gene mutations without DNA isolation and amplification using Array Piezoelectric Plate Sensor (PEPS)
@inproceedings{Kirimli2013InSD,
title={In situ detection of transrenal gene mutations without DNA isolation and amplification using Array Piezoelectric Plate Sensor (PEPS)},
author={Ceyhun E. Kirimli},
year={2013}
}In situ detection of transrenal gene mutations without DNA i solation and amplification using Array Piezoelectric Plate Sensor (PEPS). Ceyhun E. Kirimli Wan Y. Shih, Ph. D. Wei-Heng Shih, Ph. D. Low molecular weight (LMW) DNA fragments from distant organ s can pass through kidneys and exist in urine as ”transrenal DNA”. Transrenal D NA provides a noninvasive means to access cell-free DNAs from distant organs such as li ver, pancreas, colon that are otherwise difficult to obtain. Shih and Shih’s…
Figures and Tables from this paper
figure 1.1 
figure 1.10 
figure 1.11 
figure 1.12 
figure 1.13 
figure 1.14 
figure 1.2 
figure 1.3 
figure 1.4 
figure 1.5 
figure 1.6 
figure 1.7 
figure 1.8 
figure 1.9 
figure 2.1 
table 2.1 
figure 2.10 
figure 2.11 
figure 2.12 
figure 2.13 
figure 2.14 
figure 2.15 
figure 2.16 
figure 2.2 
figure 2.3 
figure 2.4 
figure 2.5 
figure 2.6 
figure 2.7 
figure 2.8 
figure 2.9 
figure 3.1 
table 3.1 
figure 3.2 
figure 3.3 
figure 3.4 
figure 3.5 
figure 3.6 
figure 3.7 
figure 3.8 
figure 3.9 
figure 4.1 
figure 4.10 
table 4.1 
figure 4.11 
figure 4.12 
figure 4.13 
figure 4.14 
figure 4.2 
table 4.2 
figure 4.3 
figure 4.4 
figure 4.5 
figure 4.6 
figure 4.7 
figure 4.8 
figure 4.9 
figure 5.1 
figure 5.2 
figure 5.3 
figure 5.4 
figure 5.5 
figure 5.6 
figure 5.7 
figure 5.8
One Citation
Piezoelectric plate sensor for in situ genetic detection of hepatitis b virus in serum without DNA isolation and amplification
- Biology
- 2013
A new type of sensor developed in Shih’s laboratory that is unique is that the shift is caused by the stress generated by the binding of the target and is inherently amplified by the polarization switching in the PMN-PT layer.
References
SHOWING 1-10 OF 193 REFERENCES
Real-time, in situ DNA hybridization detection with attomolar sensitivity without amplification using (pb(Mg1/3Nb2/3)O3)0.65-(PbTiO3)0.35 piezoelectric plate sensors.
- BiologyBiosensors & bioelectronics
- 2013
PEMC-based method of measuring DNA hybridization at femtomolar concentration directly in human serum and in the presence of copious noncomplementary strands.
- BiologyAnalytical chemistry
- 2007
Piezoelectric-excited, millimeter-sized cantilever (PEMC) sensors having high-mode resonance near 1 MHz are shown to exhibit mass change sensitivity of 1-300 ag/Hz. Gold-coated PEMC sensors…
Transrenal DNA as a Diagnostic Tool: Important Technical Notes
- BiologyAnnals of the New York Academy of Sciences
- 2004
The sensitivity and specificity of detection of mutant sequences are reduced in the presence of high excess of a respective wild‐type allele, but they can be significantly increased through application of enriched polymerase chain reaction (PCR), peptide nucleic acid (PNA) clamped PCR, and/or stencil‐aided mutation analysis (SAMA), based on selective pre‐PCR elimination of wild‐ type sequences.
Detection of the factor V Leiden mutation by direct allele-specific hybridization of PCR amplicons to photoimmobilized locked nucleic acids.
- Biology, MedicineClinical chemistry
- 1999
The simplicity of the assay and its procedural relatedness to the widely used ELISA format should make it useful for routine factor V testing in the clinical laboratory.
Transrenal DNA testing: progress and perspectives
- Biology, MedicineExpert review of molecular diagnostics
- 2006
The Tr-DNA test is expected to have utility in treatment monitoring, transplantation monitoring, drug development and broad public health screening, where a noninvasive, common-platform diagnostic technology has particular value.
QCM detection of DNA targets with single-base mutation based on DNA ligase reaction and biocatalyzed deposition amplification.
- Biology, ChemistryBiosensors & bioelectronics
- 2007
A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7.
- Biology, ChemistryBiosensors & bioelectronics
- 2006
Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors.
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
- 2006
Implementation of label-free electronic detection assays using NTNFETs constitutes an important step toward low-cost, low-complexity, highly sensitive and accurate molecular diagnostics.
Attomole detection of mesophilic DNA polymerase products by nanoparticle-enhanced surface plasmon resonance imaging on glassified gold surfaces.
- Biology, ChemistryJournal of the American Chemical Society
- 2010
A novel method that couples a template-directed polymerase extension of a surface array element with nanoparticle-enhanced detection of the reaction product is described, allowing for the detection of a specific DNA target that is present in low amounts in a sample and with partially unknown sequence.
Disposable magnetic DNA sensors for the determination at the attomolar level of a specific enterobacteriaceae family gene.
- Biology, ChemistryAnalytical chemistry
- 2008
The developed method was used for the analysis of Escherichia coli DNA fragments in polymerase chain reaction (PCR) amplicons extracted from a cell culture, and as low as 2.5 aM asymmetric PCR product could be detected with the developed methodology.