In situ detection of mature microRNAs by labeled extension on ultramer templates.

@article{Nuovo2009InSD,
  title={In situ detection of mature microRNAs by labeled extension on ultramer templates.},
  author={Gerard J Nuovo and Eun Joo Lee and Sean Lawler and Jakub Godlewski and Thomas D. Schmittgen},
  journal={BioTechniques},
  year={2009},
  volume={46 2},
  pages={115-26}
}
We describe a new method for the in situ detection of a mature microRNA (miRNA) in formalin-fixed, paraffin-embedded tissues. The method involves the labeled extension of miRNA hybridized to an approximately 100-nucleotide-long ultramer template containing the complementary sequence of the miRNA at its 3' terminus. Pretreatment of the tissue involves incubation with protease to expose the genomic DNA to DNase digestion, thereby eliminating the ultramer-independent DNA synthesis process inherent… CONTINUE READING