In situ detection of human immunodeficiency virus (HIV) nucleic acid in H9 cells using nonradioactive DNA probes and an image cytophotometry system.

Abstract

Rapid and sensitive nonradioactive methods to detect human immunodeficiency virus (HIV)-infected cells are needed in clinical medicine. We developed an in situ hybridization test using 2-acetylaminofluorene (AAF)-labeled HIV DNA as a hybridization probe. Hybridized probe was detected using rabbit anti-AAF antibody, followed by alkaline phosphatase… (More)

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@article{Donovan1988InSD, title={In situ detection of human immunodeficiency virus (HIV) nucleic acid in H9 cells using nonradioactive DNA probes and an image cytophotometry system.}, author={Ryan M. Donovan and Stuart H. Cohen and Wolter Peterson and V D Bolton and George W. Jordan and James Reynolds Carlson and K M vanden Brink and Eileen Goldstein and Christen Bush}, journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}, year={1988}, volume={36 12}, pages={1573-7} }