In situ detection of expression of thegus reporter gene in transgenic plants: ten years of blue genes

@article{Guivarch2005InSD,
  title={In situ detection of expression of thegus reporter gene in transgenic plants: ten years of blue genes},
  author={Anne Guivarc'h and J. C. Caissard and Abdelkrim Azmi and Taline Elmayan and Dominique Chriqui and Mark Tepfer},
  journal={Transgenic Research},
  year={2005},
  volume={5},
  pages={281-288}
}
Among the methods now available to localize the sites of gene expression in plant materials, reporter genes based on thegus (uidA) gene ofEscherichia coli, which encodes a β-glucuronidase (E.C. 3.2.1.31; GUS), have been the most widely used during the last ten years. The apparent simplicity of the histochemical GUS assay has been a major factor in the increase in articles usinggus genes. However, over the last four years, there have been occasional reports expressing doubts concerning the… CONTINUE READING
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