In situ detection of a PCR-synthesized human pancentromeric DNA hybridization probe by color pigment immunostaining: application for dicentric assay automation.

@article{Kolanko2000InSD,
  title={In situ detection of a PCR-synthesized human pancentromeric DNA hybridization probe by color pigment immunostaining: application for dicentric assay automation.},
  author={Christopher J Kolanko and Marla Pyle and Joginder Nath and Pataje G. S. Prasanna and H A Loats and William F. Blakely},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={2000},
  volume={75 2},
  pages={91-8}
}
We report a low cost and efficient method for synthesizing a human pancentromeric DNA probe by the polymerase chain reaction (PRC) and an optimized protocol for in situ detection using color pigment immunostaining. The DNA template used in the PCR was a 2.4 kb insert containing human alphoid repeated sequences of pancentromeric DNA subcloned into pUC9… CONTINUE READING