In situ compartmentation of creatine kinase in intact sarcomeric muscle: The acto-myosin overlap zone as a molecular sieve

@article{Wegmann1992InSC,
  title={In situ compartmentation of creatine kinase in intact sarcomeric muscle: The acto-myosin overlap zone as a molecular sieve},
  author={Gabi Wegmann and Else Zanolla and Hans M. Eppenberger and Theo Wallimann},
  journal={Journal of Muscle Research & Cell Motility},
  year={1992},
  volume={13},
  pages={420-435}
}
Creatine kinase isoenzymes (CK = ATP: creatine N-phosphoryl transferase, EC 2.7.3.2) were localizedin situ in cryosections of intact sarcomeric muscle by immunocytochemical staining. Similar to cardiac muscle, spermatozoa and photoreceptor cells, mitochondrial-type CK (Mi-CK) localization in skeletal muscle was also restricted to mitochondria. Besides the well-documented localization of muscle-type (M-CK) at the M-line and at the sarcoplasmic reticulum, surprisingly, most of the sarcoplasmic M… CONTINUE READING