In situ characterisation of a microorganism surface by Raman microspectroscopy: the shell of Ascaris eggs

@article{Quils2006InSC,
  title={In situ characterisation of a microorganism surface by Raman microspectroscopy: the shell of Ascaris eggs},
  author={Fabienne Quil{\`e}s and Jean-Yves Balandier and Sandrine Capizzi-Banas},
  journal={Analytical and Bioanalytical Chemistry},
  year={2006},
  volume={386},
  pages={249-255}
}
Intestinal nematodes are very common human parasites and a single species, Ascaris lumbricoïdes, is estimated to infect a quarter of the world’s population. A sticky external layer covers their eggs. This work shows that Raman vibrational confocal spectroscopy is able to give information on the biochemical composition of the shell of Ascaris eggs. The biochemical localised characterisation of Ascaris eggs was performed directly on the eggs in their aqueous environment. The studied parasites… 

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References

SHOWING 1-10 OF 23 REFERENCES

Ultrastructure of eggs of Ascaris lumbricoides Linnaeus, 1758. I. Egg-shells.

Under the light microscope the chitin-protein layer of egg-shells in ascarids appears to be a regular, hyaline and nonstructural layer of 1.5 to 2.00 microns in thickness, which shows a distinct lamellate structure only after a prolonged fixation with osmium at higher temperature.

Visualization of chitin-protein layer formation in Ascaris lumbricoides egg-shells.

Intensive formation of chitin structures in A. lumbricoides egg-shell occurred only in fertilized females in a very short portion of uterus from the site of connection of the oviduct with the uterus up to 25-30 mm distally, i.e. in the section forming about one fifth or less than one sixth of length of adult female uterus.

Multidimensional information on the chemical composition of single bacterial cells by confocal Raman microspectroscopy.

The multidimensional information in Raman spectra gives a global view on all major components of the cell at once, complementing other more specific information-rich methods for single-cell analysis and can be used, for example, to study heterogeneities in a microbial population.

Raman microscopic analysis of single microbial cells.

The utility of the Raman confocal microscope to generate a spectral profile from a single microbial cell and the use of this approach to differentiate bacterial species are demonstrated and suggest that Raman microscopy has significant potential for studies requiring the taxonomic identity and functioning of single microbial cells to be determined.

Embryonic and post-embryonic changes in the lipids of Ascaris lumbricoides eggs.

  • D. Fairbairn
  • Biology
    Canadian journal of biochemistry and physiology
  • 1955
Unembryonated ascaris eggs contained amounts of saponifiable and unsaponifiable lipids estimated to exceed 50% of the protoplasmic solids, and death of the embryos coincided with failure to utilize the remaining saponifiables.

Ascaris and ascariasis.

  • D. Crompton
  • Medicine, Biology
    Advances in parasitology
  • 2001

Structure of the ascarosides from Ascaris suum.

Six glycosides have been identified from the nematode Ascaris suum, and although 6 and 7 are related to ascaroside A, previously isolated from P. equorum, these earlier reports suggest the chain in ascarOSide A to be unbranched.

Structural analysis of heparin by raman spectroscopy.

The usefulness of Raman spectroscopy is extended to include structural details required for the quality assurance of pharmaceutical preparations of heparin to determine the relative proportion of the N-sulfate, 6-O-solfate, and 2-O'sulfate groups in theheparin molecule.