In situ calibration of fura-2 and BCECF fluorescence in adult rat ventricular myocytes.

@article{Borzak1990InSC,
  title={In situ calibration of fura-2 and BCECF fluorescence in adult rat ventricular myocytes.},
  author={S Borzak and Ralph A. Kelly and Bernard K Kr{\"a}mer and Yayoi Matoba and J. F. David Marsh and Martin Reers},
  journal={The American journal of physiology},
  year={1990},
  volume={259 3 Pt 2},
  pages={H973-81}
}
Quantitation of Ca+ and H+ activities within cells using presently available fluorescent probes is optimal when the fluorescence signal is calibrated in situ after each experiment. Fura-2 and 2',7'-bis(2-carboxy-ethyl)-5,6-carboxyfluoroscein (BCECF) are difficult to calibrate in freshly dissociated adult cardiac myocytes because calibration procedures produce cellular hypercontracture. In situ calibration was accomplished in rat ventricular cells by saturating fura-2 with La3+, an agent known… CONTINUE READING

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