In situ bisulfite modification of membrane-immobilized DNA for multiple methylation analysis.

@article{Wang2006InSB,
  title={In situ bisulfite modification of membrane-immobilized DNA for multiple methylation analysis.},
  author={Yan Wang and Wenli Zheng and Junfeng Luo and Dingdong Zhang and Lu Zuhong},
  journal={Analytical biochemistry},
  year={2006},
  volume={359 2},
  pages={183-8}
}
Increasing interest in DNA methylation has resulted in the recent development of array-based methods. However, these method require complex sample treatment processes, such as bisulfite modification in the liquid state of every sample. Here, we describe a microarray-based technology for interrogating DNA methylation status of multiple DNA samples at the same time, in which the DNA samples are first dotted on membranes and then treated with bisulfite directly. In this assay, plasmid pUC19 DNA is… CONTINUE READING