In situ analysis of human menin in normal and neoplastic pancreatic tissues: evidence for differential expression in exocrine and endocrine cells.

@article{Cavallari2003InSA,
  title={In situ analysis of human menin in normal and neoplastic pancreatic tissues: evidence for differential expression in exocrine and endocrine cells.},
  author={Ilaria Cavallari and D M D'agostino and Tiziana Ferro and Antonio Rosato and Luisa Barzon and Claudio Pasquali and Paola Fogar and Marily Theodoropoulou and Giovanni Esposito and Marco Boscaro and Uberto Pagotto and Elisabetta Tebaldi and Francesco Fallo and Luigi Chieco-Bianchi and Vincenzo Ciminale},
  journal={The Journal of clinical endocrinology and metabolism},
  year={2003},
  volume={88 8},
  pages={3893-901}
}
Multiple endocrine neoplasia type 1 (MEN1) is a hereditary syndrome linked to mutations in the MEN1 gene, which encodes a 610-amino-acid nuclear protein termed menin. Because of the lack of a suitable detection protocol, the in situ expression pattern of menin in human tissues remains to be determined. In this study, we have developed an antimenin monoclonal antibody and an indirect immunofluorescence/laser-scanning microscopy protocol for analyzing menin expression in frozen tissue sections… CONTINUE READING